TOWN Ladies were dumped out of this cup having won the original encounter seven days earlier.
But the fixture had to be replayed after use of repeated substitutions by both teams during the final half-an-hour of the first game led to the Essex County Football Association instructing the teams to play again with a new referee and under the correct competition rules.
It was a decision that came back to bite Brentwood as an abject performance saw the club lose for the first time in a month and also for the first time in six away matches following two draws and three consecutive wins.
The match started badly for Blues as they conceded in just the third minute when Georgia Box was in position to stab home from close range.
Although playing nowhere near their best, Town were able to fashion a number of half-chances including one when Alex Shepard combined with Hannah Porter but she fired over.
Blues did equalise when Nikita Whinnett's simple pass picked out Lucy Faraday who scored with a 35-yard strike.
Two minutes later the home side restored their lead through Annie Hickey with an angled header.
Goalmouth opportunities were restricted in the second half with Porter heading a Jo Sibley corner wide and Shepard drawing a save from Prior besides her right-hand post for the hosts, and Cristina Fragnito nodding a Faraday flag-kick wide for Blues.
Then, with a quarter-of-an-hour remaining, Colchester struck a blow to Brentwood's hopes with a third goal.
It came at the end of a prolonged period of possession when Kerry Jacks squared to Powels, who fired in via the underside of the crossbar from 20 yards out.
The former Colchester United youngster was so overjoyed with her goal that she was cautioned for her goal celebration straight afterwards.
Brentwood had to score next, but instead Town extended their advantage through the dominant Porter.
Shepard's left-wing cross was cut out by Dimmock, but it fell to midfielder Porter, who scooped over Prior from just outside the penalty area.